Table 1

Ligand affinity parameters for WT and Ser3.36Ala 5-HT2A receptors

	K_i
	WT	WT S3.36A	Ratio
	nM
5-HT	267 ± 86	7,460 ± 1,112	27.9
N-Me-5-HT	197 ± 19	1,589 ± 145	8.1
N,N-dMe-5-HT	162 ± 14	645 ± 88	4
5-MeOT	183 ± 16	3,631 ± 295	19.8
N-Me-5-MeOT	137 ± 35	748 ± 126	5.5
N,N-dMe-5-MeOT	121 ± 17	554 ± 90	4.6
4-HT	673 ± 106	6,085 ± 745	9
N,N-dMe-4-HT	95 ± 13	360 ± 76	3.8
TRYP	960 ± 133	11,250 ± 1,451	11.7
N-Me-TRYP	509 ± 56	2560 ± 452	5
N,N-dMe-TRYP	366 ± 41	982 ± 130	2.7
LSD	0.38 ± 0.05	0.54 ± 0.13	1.4
Lisuride	0.59 ± 0.01	0.58 ± 0.03	1
BOL	0.22 ± 0.01	0.38 ± 0.01	1.7
Ergonovine	0.76 ± 0.02	1.7 ± 0.2	2.2
Methysergide	6.7 ± 0.3	11.3 ± 0.5	1.7
Mesulergine	23 ± 2	38 ± 2	1.7

Values for K _i were determined from [³H]ketanserin competition binding curves. Ratio =K _i for S3.36A/K _i for WT. Data are mean ± S.E. from 3–14 experiments.