Table 1

Competition for radioligand binding to HEK 293 cell membranes expressing recombinant human (h) or rat (r) prostanoid receptors by EP4A

Prostanoid ReceptorKi
μM
hEP1 19, 17
hEP2 19, 23
hEP3   1.90  ± 0.31  (n  = 5)
hEP4 0.024  ± 0.001  (n  = 5)
hDP5.10  ± 0.41  (n  = 4)
hFP5.63  ± 0.70  (n  = 4)
hIP6.74  ± 0.66  (n  = 4)
hTP0.71  ± 0.05  (n  = 4)
rEP1 61
rEP2 58
rEP3 7.1, 7.4
rEP4 0.032

Equilibrium competition binding assays were performed as described under Materials and Methods. K i values are mean ± S.E.M. with the number of determinations shown in parentheses. Whenn = 1 or 2, the individual values are shown.