Table 1

Rhodopsin helix-helix interactions found experimentally.

Residue Indices	Experimental Methods		Residues/Mutations	Cβ-Cβ	References
Residue Indices	C	R	Residues/Mutations	Cβ-Cβ	References	Z	E	L	F
								Å
1.60 7.63	⁺			⁺			H65C C316	5.3	Yang et al., 1996; Struthers et al., 2000
2.57 7.43		⁺					G90D K296	8.3^1-a	Oprian, 1992; Rao et al., 1994
3.28 7.43					⁺		E113 K296	10.1	Oprian, 1992; Rao et al., 1994
3.36 5.22	⁺						C110 C187	12.9	Struthers et al., 2000
3.36 6.44		⁺					G121L F261A	9.0	Han et al., 1996; Lin et al., 2000
3.37 5.46						⁺	E122 H211	6.1	Beck et al., 1998
3.51 5.57	⁺						Y136C C222	5.1	Yu and Oprian, 1999
3.51 5.60	⁺						Y136C C225	6.0	Yu and Oprian, 1999
3.53 6.34			⁺				V138H T251H	8.3	Sheikh et al., 1996
3.54 5.60			⁺				V139C Q225C	8.6	Yu and Oprian, 1999
3.54 6.30	⁺						V139C E247C	6.3	Farrens et al., 1996
3.54 6.31	⁺			⁺			V139C K248C	8.2	Farrens et al., 1996
3.54 6.32	⁺			⁺			V139C E249C	11.8	Farrens et al., 1996
3.54 6.33	⁺			⁺			V139C V250C	8.9	Farrens et al., 1996
3.54 6.34	⁺			⁺			V139C T251C	5.7	Farrens et al., 1996
3.54 6.35				⁺			V139C R252C	10.8	Farrens et al., 1996
3.55 5.57	⁺						C140 C222	8.3	Farrens et al., 1996; Yu and Oprian, 1999
3.55 5.60	⁺						C140 Q225C	4.9	Yu and Oprian, 1999; Struthers et al., 2000
3.55 7.63	⁺						C140 C316	28.5^1-a	Yu et al., 1999
5.33 6.59	⁺						T198C F276C	14.5	Struthers et al., 1999
5.35 6.59	⁺						N200C F276C	9.0	Struthers et al., 1999
5.39 6.59	⁺						V204C F276C	5.1	Struthers et al., 1999, 2000

Cβ-Cβ is the distance between the Cβ of two residues in the bovine rhodopsin structure. Receptor names are abbreviated according to their SWISS-PROT Annotated Protein Sequence Database entry names:http://www.expasy.ch/cgi-bin/lists?7trmrlist.txt

C, Cys-Cys crosslinking; R, double revertant mutations; Z, Zinc-His binding; E, spin-labeling; L, ligand related; F, Fourier-transform infrared difference spectroscopy.
↵1-a Present only in the active state.