Strain | Ligand | |||||
---|---|---|---|---|---|---|
[3H]RX821002 | [3H]Rauwolscine | [3H]Rauwolscine + 100 nM oxymetazoline | ||||
OE | OE-wt | OE | OE-wt | OE | OE-wt | |
fmol/mg of tissue protein | ||||||
Male mice | 133 ± 4 | 60 ± 2 | 157 ± 9 | 62 ± 1 | 120 ± 11 | 39 ± 4 |
Female mice | 191 ± 8 | 75 ± 10 | 194 ± 11 | 76 ± 4 | 98 ± 15 | 34 ± 6 |
[3H]RX821002 was used to detect total α2-AR binding. The results obtained with the α2C-AR-preferring radioligand [3H]rauwolscine, in the presence of 100 nm oxymetazoline to block α2A-ARs, reflect α2C-AR density.
Values are mean ± standard error for four measurements.