Phosphoinositide response to acetylcholine of m1 mAChRs mutated at Asp122 or Tyr124
| Mutant | pEC50 | Emax |
|---|---|---|
| −log (m) | % basal | |
| Wild-type | 7.1 ± 0.13-a | 280 ± 20 |
| D122E | 7.0 ± 0.2 | 260 ± 40 |
| D122N | 6.9 ± 0.2 | 220 ± 30 |
| D122H | 6.0 ± 0.1 | 270 ± 50 |
| D122A | 0 | |
| D122S | 5.8 | 40 |
| D122C | 0 | |
| D122L | 0 | |
| D122K | 0 | |
| Y124F | 7.0 ± 0.1 | 300 ± 20 |
| Y124W | 6.5 ± 0.1 | 340 ± 60 |
| Y124H | 6.2 ± 0.2 | 250 ± 60 |
| Y124M | 6.1 ± 0.4 | 240 ± 50 |
| Y124C | 5.8 ± 0.3 | 240 ± 30 |
| Y124I | 0 | |
| Y124R | 0 | |
| Y124E | 0 |
↵3-a Values are mean ± standard error of three or more observations.