Subunit combination | IC_{50} at −70 mV^{2-a} | K_{d} (0)^{2-b} | zδ^{2-b} | No. of oocytes |
---|---|---|---|---|
μm | μm | |||
Wild-type NR1/NR2A | 0.38 | 779 ± 272 | 2.58 ± 0.10 | 14 |
NR1(N616Q)/NR2A | 5.7 | 1351 ± 170^{2-c} | 1.81 ± 0.04^{2-d} | 21 |
NR1(N616G)/NR2A | 0.01 | 45 ± 7^{2-c} | 2.71 ± 0.04 | 15 |
NR1/NR2A(N615G) | 0.02 | 29 ± 5^{2-c} | 2.59 ± 0.06 | 12 |
↵2-a Data for the IC_{50} values of N^{1}-DnsSpm are from Table 4.
↵2-b Values for K_{d} (0) and zδ were derived using text eq. 2 with data from voltage ramps (−100 to +40 mV over 6 or 12 sec or −150 to +40 mV over 6 sec), using data from the part of the I-V relationship that showed a steep voltage dependence. The concentrations of N^{1}-DnsSpm that were used were 0.3–2 μM for NR1/NR2A, 2 or 30 μm for NR1(N616Q)/NR2A, and 0.1 μm for NR1(N616G)/NR2A and NR1/NR2A(N615G). For NR1(N616Q)/NR2A the values ofK_{d} (0) (1597 ± 279 μm) and zδ (1.73 ± 0.03) determined in experiments with 30 μm N^{1}-DnsSpm using ramps from −100 to +40 mV were similar to the values of K_{d} (0) (1128 ± 190 μm) and zδ (1.88 ± 0.06) determined in experiments with 2 μm N^{1}-DnsSpm using ramps from −150 to +40 mV.
↵2-c p < 0.05,
↵2-d p < 0.01 compared with wild-type NR1/NR2A (one-way ANOVA with post hoc Dunnett’s test).