Subunit Combination | N1-DnsSpm IC50 | nH | Mutant/wild-type4-a | No. of oocytes |
---|---|---|---|---|
μm | ||||
NR1/NR2A series | ||||
Wild-type NR1/NR2A | 0.38 ± 0.11 | 0.9 ± 0.1 | 9 | |
NR1(N616Q)/NR2A | 5.7 ± 0.84-b | 0.7 ± 0.1 | 15 | 6 |
NR1(N616R)/NR2A | 79 ± 8.24-b | 1.0 ± 0.1 | 208 | 6 |
NR1(N616G)/NR2A | 0.01 ± 0.0014-b | 0.9 ± 0.1 | 0.03 | 6 |
NR1/NR2A(N614Q) | 0.09 ± 0.034-b | 1.1 ± 0.1 | 0.23 | 5 |
NR1/NR2A(N615Q) | 1.2 ± 0.24-b | 0.9 ± 0.1 | 3 | 5 |
NR1/NR2A(N615G) | 0.02 ± 0.0024-b | 0.8 ± 0.1 | 0.05 | 4 |
NR1(N616Q)/NR2A(N615Q) | 0.19 ± 0.034-c | 0.8 ± 0.1 | 0.5 | 5 |
NR1(N616Q)/NR2A(N615G) | 0.01 ± 0.0014-b , 4-c | 1.0 ± 0.1 | 0.03 | 5 |
NR1/NR2B series | ||||
Wild-type NR1/NR2B | 0.40 ± 0.07 | 1.3 ± 0.1 | 5 | |
NR1(N616Q)/NR2B | 5.7 ± 1.24-b | 0.6 ± 0.1 | 14 | 6 |
NR1/NR2B(N615Q) | 0.14 ± 0.02 | 1.1 ± 0.1 | 0.35 | 5 |
NR1/NR2B(N616Q) | 3.2 ± 1.64-b | 1.1 ± 0.1 | 8 | 6 |
NR1(N616Q)/NR2B(N615Q) | 0.07 ± 0.014-b , 4-c | 1.0 ± 0.1 | 0.2 | 5 |
NR1(N616Q)/NR2B(N616Q) | 0.25 ± 0.034-c | 0.9 ± 0.1 | 0.6 | 5 |
Values for the IC50 and Hill slope (nH ) of inhibition by N1-DnsSpm were determined from concentration-inhibition curves using oocytes expressing NR1/NR2 receptors and voltage-clamped at −70 mV. Values are mean ± standard error.