Table 1

Primers used for PCR cloning of OCT1 cDNA

Primer designationPrimer sequence
Primer 15′-ATGCCCACCGTGGATGATGTCCTGG-3′
Primer 25′-AGTGCGGAACAGGTC-3′
5′-RACE-15′-AGCCGGGCGTGTCATACACCCA-3′
5′-RACE-25′-AGCGGCAGGTGGCTCCTGTTGG-3′
5′-RACE-35′-CACTGTATAGTTCAGCTCCTCC-3′
Anchor primer 11-a(5′-RACE)5′-CUACUACUACUAGGCCACGTCGACTAGTACGGGIIGGGIIGGGIIG-3′
Anchor primer 21-a(5′-RACE)5′-CUACUACUACUAGGCCACGCGTCGACTAGTAC-3′
3′-RACE-15′-TACTACTGGTGTGTGCCGGAG-3′
3′-RACE-25′-ATCGCTCAAAAGAATGGGAAG-3′
Adapter primer1-a(3′-RACE)5′-GGCCACGCGTCGACTAGTACTTTTTTTTTTTTTTTTT-3′
Amplification primer1-a(3′-RACE)5′-GGCCACGCGTCGACTAGTAC-3′
5′-End primer5′-GGAATTCCATCATGCCCACCGTGGA-3′       EcoRI site
3′-End primer5′-GCTCTAGAGCAAAACATCTCTCTCA-3′       XbaI site

Restriction enzyme recognition sites incorporated into primer sequences are indicated by underlining.

    • ↵1-a These primers were provided with the 5′- and 3′-RACE system (GIBCO BRL).