Primer designation | Primer sequence |
---|---|
Primer 1 | 5′-ATGCCCACCGTGGATGATGTCCTGG-3′ |
Primer 2 | 5′-AGTGCGGAACAGGTC-3′ |
5′-RACE-1 | 5′-AGCCGGGCGTGTCATACACCCA-3′ |
5′-RACE-2 | 5′-AGCGGCAGGTGGCTCCTGTTGG-3′ |
5′-RACE-3 | 5′-CACTGTATAGTTCAGCTCCTCC-3′ |
Anchor primer 11-a(5′-RACE) | 5′-CUACUACUACUAGGCCACGTCGACTAGTACGGGIIGGGIIGGGIIG-3′ |
Anchor primer 21-a(5′-RACE) | 5′-CUACUACUACUAGGCCACGCGTCGACTAGTAC-3′ |
3′-RACE-1 | 5′-TACTACTGGTGTGTGCCGGAG-3′ |
3′-RACE-2 | 5′-ATCGCTCAAAAGAATGGGAAG-3′ |
Adapter primer1-a(3′-RACE) | 5′-GGCCACGCGTCGACTAGTACTTTTTTTTTTTTTTTTT-3′ |
Amplification primer1-a(3′-RACE) | 5′-GGCCACGCGTCGACTAGTAC-3′ |
5′-End primer | 5′-GGAATTCCATCATGCCCACCGTGGA-3′ EcoRI site |
3′-End primer | 5′-GCTCTAGAGCAAAACATCTCTCTCA-3′ XbaI site |
Restriction enzyme recognition sites incorporated into primer sequences are indicated by underlining.
↵1-a These primers were provided with the 5′- and 3′-RACE system (GIBCO BRL).