Table 2

Effects of TZM on DNA content and cell volume of OGAT− and OGAT+ leukemic cells

Cell lineTZM concentrationCells with reduced DNA contentCells with reduced volume
μm %
K562 OGAT−5  ± 15  ± 1
12.511  ± 210  ± 1
2525  ± 125  ± 3
5036  ± 439  ± 5
K562 OGAT+5  ± 23  ± 2
12.54  ± 15  ± 1
255  ± 27  ± 1
5014  ± 114  ± 2
U937 OGAT−5  ± 23  ± 2
12.511  ± 115  ± 2
2528  ± 229  ± 3
5043  ± 239  ± 2
U937 OGAT+3  ± 22  ± 3
12.54  ± 13  ± 1
255  ± 15  ± 2
5011  ± 111  ± 2

Cells were exposed to graded concentrations of TZM and after 72 hr analyzed by flow cytometry as described in Materials and Methods. Values represent the mean ± standard error of three independent experiments. Data represent the percentage of cells characterized by a broad hypodiploid peak, typical of apoptosis. Values represent the mean ± standard error of three independent experiments. Data represent the percentage of cells characterized by reduced cell size compared with untreated controls, as assessed by forward scatter analysis.