Cell treatment | Specific activity pmol/min/mg | |
---|---|---|
HO | Biliverdin reductase | |
Control | 2.5 ± 0.7 | 68.1 ± 8.4 |
Hemin (6 hr) | 10.2 ± 1.01-a | 63.6 ± 7.2 |
Cell lysates prepared from control or hemin-treated cells (100 μm for 6 hr) were incubated with 2 μm hemin or biliverdin in the presence of 1 mm NADPH for determination of HO or biliverdin reductase activity, respectively. Bilirubin formation was quantified by HPLC as described in Experimental Procedures. Values are mean ± standard deviation of three (HO) or four (biliverdin reductase) individual experiments.
↵1-a Significantly different from control (p < 0.01).