Table 1

ELISA detection of human P2Y1 receptor mutants on the surface of COS-7 cells.

ConstructExpression
%
Wild-type100
COS-7 (control)  0
R128A263  ± 4
F131A150  ± 96
H132A57.2  ± 4.7
Y136A50.6  ± 32.5
T221A87.2  ± 16.9
T222A123  ± 31
F226A144  ± 42
H277A129  ± 6
K280A148  ± 13
Q307A151  ± 9
R310A132  ± 9
R310K188  ± 40
R310S/S314R114  ± 5
S314A125  ± 5
S314T111  ± 56
S317A106  ± 23

Data are presented as means ± standard deviation of eight independent determinations. Table shows expression level as percentage of Ha-tagged P2Y1 wild-type (100%). Expression level was determined using a standard curve (5).