ELISA detection of human P2Y1 receptor mutants on the surface of COS-7 cells.
| Construct | Expression |
|---|---|
| % | |
| Wild-type | 100 |
| COS-7 (control) | 0 |
| R128A | 263 ± 4 |
| F131A | 150 ± 96 |
| H132A | 57.2 ± 4.7 |
| Y136A | 50.6 ± 32.5 |
| T221A | 87.2 ± 16.9 |
| T222A | 123 ± 31 |
| F226A | 144 ± 42 |
| H277A | 129 ± 6 |
| K280A | 148 ± 13 |
| Q307A | 151 ± 9 |
| R310A | 132 ± 9 |
| R310K | 188 ± 40 |
| R310S/S314R | 114 ± 5 |
| S314A | 125 ± 5 |
| S314T | 111 ± 56 |
| S317A | 106 ± 23 |
Data are presented as means ± standard deviation of eight independent determinations. Table shows expression level as percentage of Ha-tagged P2Y1 wild-type (100%). Expression level was determined using a standard curve (5).