Effect of mecamylamine and d-tubocurarine on (−)-nicotine-evoked up-regulation of α4β2 nAChRs in HEK 293 cells
| Treatment | Condition | |
|---|---|---|
| Absence of nicotine | Presence of nicotine | |
| % over control | ||
| Control | 100 | 630 ± 97 |
| Mecamylamine, (100 μm) | 99 ± 5 | 671 ± 125 |
| d-Tubocurarine, (100 μm) | 171 ± 92-a | 675 ± 132 |
HEK 293 cells stably expressing human α4β2 nAChRs were treated with mecamylamine or d-tubocurarine in presence or absence of 1 μm (−)-nicotine, for 168 hr. [3H]Cytisine binding to membranes was performed as described in Materials and Methods.
↵2-a Significantly different from the corresponding controls.
Values are mean ± standard error of three observations.