Table 1

Gene expression, CENU cytotoxicity, and UV sensitivity in 14 human tumor cell lines

Cell line	ERCC2 protein	MGMT		BCNU IC₉₀	UV LD₉₀
Cell line	ERCC2 protein	Protein levels	Activity	BCNU IC₉₀	UV LD₉₀
			pmol/mg of protein		J/m²
MCF-7 (B)	0.06 ± 0.004	1	3.240	69 ± 8	12.4 ± 1.0
T98-G (G)	0.065 ± 0.012	0.306 ± 0.127	0.645	46 ± 8	9.6 ± 0.86
SKI-1 (G)	0.050 ± 0.026	0.012 ± 0.012	0.024	15 ± 1	8.9 ± 1.4
HT29 (C)	0.002 ± 0.002	0.613 ± 0.089	1.140	55 ± 3	8.9 ± 0.1
SF-295 (G)	0.081 ± 0.021	0.037 ± 0.037	0.065	23 ± 0	8.8 ± 0.7
MGR-3 (G)	0.022 ± 0.022	0.022 ± 0.022	0.038	86 ± 2	16.4 ± 1.2
SK-MG-4 (G)	0.065 ± 0.006	0.052 ± 0.025	0.070	11 ± 1	13.1 ± 1.4
CAKI-1 (R)	0.093 ± 0.026	0.393 ± 0.139	0.883	63 ± 4	7.7 ± 0.7
786-0 (R)	0.062 ± 0.035	0.023 ± 0.019	0.021	17 ± 1	10.3 ± 1.7
SK-MG-1 (G)	0.006 ± 0.004	0	0	14 ± 1	6.6 ± 0.3
SKNSH (N)	1	0.167 ± 0.094	0.435	173 ± 10	38.2 ± 1.9
UW-28 (G)	0.018 ± 0.018	0.134 ± 0.134	0.359	35 ± 6	17.6 ± 1.7
ACHN (R)	0.182 ± 0.042	0.681 ± 0.011	1.200	50 ± 5	9.2 ± 0.7
A498 (R)	0.316 ± 0.016	0.130 ± 0.083	0.155	147 ± 5	25.1 ± 2.3

ERCC2 protein level for each cell line was divided by ERCC2 protein level for SKNSH, for each experiment. The result is expressed as the mean ± standard error of at least three separate experiments from two separate total cellular protein extractions for each cell line. MGMT protein levels for each cell line was divided by the MGMT protein level for MCF-7 for each experiment. The result is expressed as the mean ± standard error of at least three separate experiments from two separate total cellular protein extracts for each cell line. Cytotoxicity to BCNU is expressed as the mean ± standard error of at least three separate experiments. UV sensitivity is expressed as the mean ± standard error of at least three separate experiments.

Types of tumor cell line: B, breast; C, colon; G, glioma; N, neuroblastoma; R, renal.