Table 1

Comparison of Kd andB max of wild-type, epitope-tagged, and GFP-fused α1-ARs.

ConstructsKd Bmax
nm pmol/mg
α1A 81.33.94
α1A-FH72.21.96
α1A-GFP80.50.88
α1A-FH-GFP48.31.58
α1B 86.62.13
α1B-GFP74.54.10

Affinity for 125I-HEAT binding was determined in membrane preparations from cultured COS-7 cells transiently transfected with constructs for epitope-tagged and GFP-fused α1-ARs. Each value is the mean of two to three different experiments.