Pattern of homozygous deletion of chromosome 9p21 markers in MTAP-transfected and control cell lines
| Cell line | Doubling time | IFNB1 | IFNA1 | MTAP | p16 3′ | p15 Exon 2 | D9S171 | D9S169 |
|---|---|---|---|---|---|---|---|---|
| hr | ||||||||
| MiaPaCa-2 WT | 23.9 | − | − | − | − | − | + | + |
| MiaPaCa-2/neo | 20.7 | − | − | − | − | − | + | + |
| MiaPaCa-2/MTAP-AzG | 26.5 | − | − | 0.30 ± 0.04 | − | − | + | + |
| MiaPaCa-2/MTAP-G | 24.2 | − | − | 0.21 ± 0.09 | − | − | + | + |
| PANC-1 WT | 31.9 | + | + | − | − | − | + | + |
| PANC-1/neo | 33.0 | + | + | − | − | − | + | + |
| PANC-1/MTAP-Az | 34.4 | + | + | 0.20 ± 0.10 | − | − | + | + |
| PANC-1/MTAP-G | 24.7 | + | + | 0.11 ± 0.02 | − | − | + | + |
All markers except MTAP were assessed by PCR amplification of genomic DNA, as described previously (24). Doubling time determined using DMEM containing 10% fetal bovine serum. MTAP was assessed using a previously described radiochemical assay (24). Enzyme activity, expressed as nmol of adenine formed/min/mg of protein, is given in parentheses (mean ± standard deviation of at least two determinations).-, MTAP activity in the cell extract was <0.01 nmol of adenine formed/min/mg of protien.
+, Expected PCR product for the particular marker was observed, indicating the presence of at least one allele of the DNA segment in question. −, No PCR product was observed, indicating a homozygous deletion.