Type of cell | Medium | Incubation | Challenge | Mean response ± SD | n |
---|---|---|---|---|---|
% of control | |||||
42G (transfected) | Ca2+-free PBS with 1 mm EGTA | 50 μm nicotine for 1 hr; 1-hr recovery | 10 μmnicotine | 2.2 ± 3.4 | 3 |
42G (transfected) | PBS with Ca2+ | 50 μmnicotine for 1 hr; 1-hr recovery | 10 μmnicotine | 2.4 ± 4.1 | 3 |
Cells were incubated with a deactivating concentration of nicotine (50 μ ( for 1 hr) with or without calcium in the incubation medium. There was no difference between the amount of deactivation achieved.