Treatment | U937/WT | U937/TAM | ||
---|---|---|---|---|
ng/106cells | rad equivalents | ng/106 cells | rad equivalents | |
Control | 269 ± 21 | 115 ± 5 | 287 ± 18 | 105 ± 5 |
SMase | 2112 ± 9254-a | 5130 ± 2614-a | 273 ± 704-b | 144 ± 364-b |
rhTNF-α | 1736 ± 2654-a | 3662 ± 1424-a | 326 ± 924-c | 127 ± 224-c |
Fas monoclonal antibody | 2469 ± 4164-a | 4812 ± 3154-a | 302 ± 1044-d | 138 ± 474-d |
In parallel trials, U937/WT cells and U937/TAM cells were treated with bacterial SMase (50 munits/ml) or exposed to rhTNF-α (100 nm) or monclonal antibody directed against Fas (1.5 μg/ml) for 9 hr; apoptotic DNA damage was then measured by quantitative spectrofluorophotometry as described in Materials and Methods.