Treatment | Control | SB-203580 | SKF-105209 |
---|---|---|---|
% | |||
Vehicle | 3 ± 1 | 4 ± 1 | 2 ± 1 |
Ceramide | 51 ± 75-a | 53 ± 25-a | 49 ± 65-a |
Sphingosine | 73 ± 65-a | 70 ± 55-a | 77 ± 45-a |
U937 cells were exposed to ceramide (10 μm) or sphingosine (10 μm) for 6 hr in the absence or presence of the selective pharmacological p38-RK inhibitor SB-213580 or the inactive analog SKF-105209. The fraction of cells exhibiting cytological evidence of apoptotic DNA damage was then determined by fluorescence microscopy as described in Materials and Methods; all values are expressed as the percentage of apoptotic cells within the total scored for a given treatment.
↵5-a Increased vs.vehicle-treated controls (p < 0.01).