Table 5
Multiple modifications
| Compound | Inhibition of tubulin polymerization | Inhibition of colchicine binding | Inhibition of MCF-7 growth |
|---|---|---|---|
| IC50 ± SD (μm) | % inhibition ± SD | IC50 ± SD (μm) | |
| Curacin A | 0.72 ± 0.2 | 94 ± 2 | 0.038 ± 0.01 |
| 15 | 0.73 ± 0.1 | 71 ± 5 | 0.61 ± 0.2 |
| 16 | 3.2 ± 0.3 | 16 ± 3 (32 ± 4) | 3.1 ± 2 |
| 17 | >80 | 7 ± 4 (2 ± 3) | >10 |
| 18 | 5.5 ± 0.7 | 2 ± 2 (39 ± 4) | >15-a |
| 19 | 1.1 ± 0.08 | 41 ± 4 | 2.2 ± 0.6 |
| 20 | 40 ± 10 | 4 ± 1 (4 ± 5) | >10 |
| 21 | 1.9 ± 0.1 | 23 ± 11 (85 ± 3) | 0.45 ± 0.09 |
In the colchicine binding assay, inhibitor concentration was 5.0 μm, except for the values in parentheses, where the inhibitor concentration was 50 μm.
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↵5-a Insufficient sample to test at higher concentrations.
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SD, standard deviation.