Table 2

Summary of voltage-clamp results

GABADiazepam potentiation
EC50 μMnHMaximum potentiationEC50nH
μM nm
α1β2γ212.7  ± 0.71.07  ± 0.051.91  ± 0.1751  ± 61.11  ± 0.10
α1β28.2  ± 0.40.93  ± 0.04
β2γ2210  ± 120.75  ± 0.031.03  ± 0.0724  ± 20.89  ± 0.06
α1β2χ16117.4  ± 1.40.93  ± 0.060.26  ± 0.01295  ± 341.41  ± 0.12
α1β2χ16714.9  ± 1.30.96  ± 0.060.28  ± 0.02324  ± 531.30  ± 0.17

Dose-response data for wild-type and chimeric subunit combinations for GABA and diazepam potentiation of GABA-mediated CI current inX. laevis oocytes are tabulated. Two-electrode voltage-clamp and data analysis was performed as described (see Materials and Methods). Mean and standard deviation values for maximum potentiation, EC50 values, and Hill coefficients (n H) were calculated from dose-response data (Figs. 4b and 5) with the use of Prism software.