Table 2

Effects of topically administered compounds on phorbol ester-induced inflammation in mouse ear

CompoundEdema at 4 hr ED50Myeloperoxidase response ED50
mg/ear mg/ear
 SB 2167540.490.48

Balb/c mice were given phorbol-12-myristate-13-acetate followed by test compound. The edematous response was measured using a thickness gauge 4 hrs after phorbol-12-myristate-13-acetate application. The animals were killed, the inflamed ears were harvested, and myeloperoxidase was extracted and assayed spectrophotometrically as described in Experimental Procedures. Both compounds produced effects significantly different from vehicle treatment.