Table 1

Reversal potential and shift in reversal potential for wild-type and mutant ρ1 GABA receptors

CombinationReversal potential1-aReversal potential shiftOocytes
mV n
ρ1−53.7  ± 6.5−2.4  ± 1.79
ρ1 (w/GABA)−29.7  ± 3.914.1  ± 4.99
ρ1L301A−31.3  ± 2.014.5  ± 3.96
ρ1L301G−31.5  ± 2.313.2  ± 0.86
ρ1L301S−30.1  ± 1.615.3  ± 2.46
ρ1L301T−29.3  ± 2.312.7  ± 4.16
ρ1L301V−33.5  ± 4.38.2  ± 1.96
ρ1L301Y−31.0  ± 1.610.5  ± 1.06

The shift in reversal potential was determined by reducing the external chloride concentration from 99 to 39 mm. Values are mean ± standard deviation.

    • 1-a Reversal potential of the wild-type receptor was determined during a steady application of 10 μm GABA as described in the text.