Ligand | Ki | |
---|---|---|
WT | W351A | |
nm | ||
Agonist | ||
CCK8 | 1.40 ± 0.16 | 8.14 ± 2.054-a |
pBC264 | 1.21 ± 0.20 | 1.61 ± 0.35 |
CCK4 | 30.3 ± 3.50 | 359 ± 414-a |
Antagonist | ||
L-365,260 | 10.9 ± 2.00 | 11.8 ± 0.1 |
PD-134,308 | 2.60 ± 0.10 | 24.9 ± 4.74-a |
L-364,718 | 276 ± 85 | 960 ± 2694-a |
Ki values were calculated from competition binding experiments using [3H]pBC264 (0.5 nm) as radioligand as described in the text. Data are expressed as the mean ± standard error of three or four experiments, each performed in triplicate.
↵4-a p < .01, Student’s ttest.