Table 1

Effect of various compounds on Mrp2-mediated [3H]E217βG transport

CompoundConcentration[3H]E217βG uptake
μm % control
None (control)100
LTC4 1022  ± 71-b
α-Naphthyl-β-d-glucuronide100094  ± 1
p-Nitrophenyl-β-d-glucuronide100097  ± 5
Phenolphthalein glucuronide10005  ± 41-b
MK571533  ± 31-a
CsA1048  ± 11-a
FL100094  ± 4
MTX100055  ± 41-a
FL-MTX10022  ± 71-b

Sf9-Mrp2 membrane vesicles were incubated with 50 nm[3H]E217βG at 37° for 1 min with or without (control) the compounds as indicated. Net ATP-dependent transport was calculated by subtracting values in the presence of 4 mm5′-AMP from those in the presence of 4 mm ATP. Transport was expressed as percent of the control uptake. Data represent mean values of three to six determinations (± standard error).

  • ↵1-a p < 0.05.

  • ↵1-b p < 0.01 (analysis of variance with Bonferroni’s correction).