Binding of [3H]flunitrazepam and [3H]Ro 15-1788 after photoincorporation of chlordiazepoxide, flunitrazepam, and analogues
| Photoincorporation condition | [3H]Flunitrazepam | [3H]Ro 15-1788 | |
|---|---|---|---|
| Flunitrazepam | B max (% of control binding) | 9.4 ± 10.5 | 76.7 ± 6 |
| +UV | K d(nm) | N.D. | 1.12 ± 0.8 |
| Des-methyl flunitrazepam | B max (% of control binding) | 22.1 ± 17 | 69.3 ± 11 |
| +UV | K d(nm) | N.D. | 0.99 ± 0.1 |
| Des-methyl, des-nitro, flunitrazepam | B max (% of control binding) | 86.1 ± 4 | 82.9 ± 12 |
| +UV | K d (nm) | 3.4 ± 1.6 | 1.02 ± 0.28 |
| Chlordiazepoxide | B max (% of control binding) | 103 ± 13 | 98.3 ± 6 |
| +UV | K d (nm) | 4.3 ± 1.2 | 0.66 ± 0.09 |
Data shown are mean ± standard error of four separate experiments in which binding was measured by saturation analysis using eight concentrations of ligand. Data are expressed as percent of binding in control membranes, in which membranes were not exposed to ultraviolet light or preincubated with any compound. Binding in control cell membranes ranged from 1055 to 1925 fmol/mg of protein in these experiments.
N.D., not detected.