Table 4

The effect of AG treatment on LPS-stimulated decrease in microsomal P450 hydroxylase activities

	Time	Saline	LPS	LPS + AG	AG
	hr	nmol/mg/min
Testosterone hydroxylation
6β	6	1.15 ± 0.03	0.85 ± 0.04^4-150	1.23 ± 0.06	1.49 ± 0.26
	24	1.52 ± 0.17	0.76 ± 0.13^4-150	0.29 ± 0.12^4-150	0.71 ± 0.09^4-150
7α	6	0.65 ± 0.01	0.58 ± 0.06	0.54 ± 0.15	0.48 ± 0.10
	24	0.55 ± 0.09	0.13 ± 0.03^4-150	0.22 ± 0.03^4-150	0.40 ± 0.06
15α	6	0.40 ± 0.05	0.22 ± 0.07	0.28 ± 0.08	0.36 ± 0.09
	24	0.36 ± 0.08	0.22 ± 0.01^4-150	0.12 ± 0.03^4-150	0.19 ± 0.02^4-150
16α	6	0.60 ± 0.03	0.44 ± 0.07	0.47 ± 0.07	0.52 ± 0.12
	24	0.19 ± 0.06	0.04 ± 0.01^4-150	0.19 ± 0.05	0.18 ± 0.07
16β	6	0.89 ± 0.09	0.84 ± 0.06	0.59 ± 0.06	0.40 ± 0.12^4-150
	24	0.82 ± 0.06	0.49 ± 0.02^4-150	0.14 ± 0.02^4-150	0.12 ± 0.01^4-150
p-Nitrophenol hydroxylation
	6	1.51 ± 0.05	1.16 ± 0.12^4-150	0.75 ± 0.11^4-150	0.91 ± 0.04^4-150
	24	1.36 ± 0.24	0.65 ± 0.07^4-150	0.98 ± 0.13	1.21 ± 0.12

Mice were injected with LPS followed by repeated injections of AG every 4 hr for 6 and 24 hr. Animals were killed at the appropriate times and hepatic microsomal protein isolated and analyzed as described in Materials and Methods. The data presented in the table represent the mean ± standard error of five mice per treatment group.

↵4-150 Significantly different from control, p < 0.05.