Drug | Time | Fraction of apoptotic cells | |
---|---|---|---|
TK6 | MT1 | ||
hr | % | ||
None | 6 | 7, 5 | 5, 4 |
TMZ, 12.5 μm | 6 | 7, 8 | 2, 6 |
TMZ, 25 μm | 6 | 7, 2 | 2, 1 |
ETO, 250 nm | 6 | 11, 9 | 3, 6 |
None | 24 | 6, 5 | 2, 3 |
TMZ, 12.5 μm | 24 | 8, 10 | 4, 3 |
TMZ, 25 μm | 24 | 14, 11 | 4, 6 |
ETO, 250 nm | 24 | 21, 27 | 21, 13 |
None | 48 | 2, 3 | 4, 3 |
TMZ, 12.5 μm | 48 | 24, 27 | 4, 3 |
TMZ, 25 μm | 48 | 32, 23 | 4, 5 |
ETO, 250 nm | 48 | 35, 49 | 39, 37 |
None | 72 | 2, 1 | 2, 2 |
TMZ, 12.5 μm | 72 | 28, 35 | 2, 1 |
TMZ, 25 μm | 72 | 45, 35 | 2, 8 |
ETO, 250 nm | 72 | 44, 50 | 37, 44 |
The percentage of apoptotic cells was evaluated by flow cytometric analysis of DNA content. Data represent the values obtained in two independent experiments. Cells were cultured in the presence of the indicated concentrations of TMZ or etoposide (ETO) for 6, 24, 48, or 72 hr and were then processed for apoptosis evaluation.