Effect of THC and other cellular modulators on p42/p44 MAPK activity in primary cultures of cortical astrocytes
| Additions | MAPK activity |
|---|---|
| pmol/min × 106cells | |
| None | 15.2 ± 3.0 |
| THC | 52.7 ± 13.33-a |
| SR 141716 + THC | 15.7 ± 3.8 |
| Pertussis toxin + THC | 17.1 ± 4.2 |
| HU-210 | 56.4 ± 15.93-a |
| Forskolin | 216.4 ± 55.83-a |
| C8-ceramide | 63.5 ± 1.73-a |
| Sphingomyelinase | 83.4 ± 12.73-a |
Astrocytes were exposed to the different agents for 10 min. In the case of incubations containing SR 141716, cells were preincubated for 30 min with this compound before THC was added. Results correspond to four different experiments for each experimental condition. Final concentrations of the different additions were: THC, 100 nm; SR 141716, 1 μm; pertussis toxin, 25 ng/ml; HU-210, 10 nm; forskolin, 20 μm; C8-ceramide, 25 μm; and sphingomyelinase, 0.2 units/ml.
↵3-a Significantly different (p < 0.01) versus incubations with no additions.