Binding and displacement parameters for rat preparations
Direct Binding | Displacement | ||||||||
---|---|---|---|---|---|---|---|---|---|
Nicotine | Cytisine | Ligand | CytisineKi | Nicotine Ki | dHβEKi | Ref.1-a | |||
Bmax | K | Bmax | K | ||||||
fmol/mg | nM | fmol/mg | nM | nM | nM | nM | |||
Rat brain (P2) | 115 | 0.9 | 99 | 0.20 | Cytisine | 0.14 | 0.7 | 17 | 1 |
Rat cortex | – | – | 4 | 0.90 | Cytisine | 0.45 | 3.3 | 22 | 2 |
Rat cortex | 57 | 3.5 | – | – | Nicotine | 2.00 | 4.2 | 91 | 3 |
Rat spinal cord | 20 | 2.2 | 20 | 0.40 | Cytisine | 0.71 | 2.3 | 21 | 4 |
Rat mean | 2.2 | 0.50 | 0.83 | 2.6 | 38 | ||||
(S.D.) | (1.1) | (0.29) | (0.71) | (1.3) | (31) | ||||
HN42 cells | 544 | 0.40 | 383 | 0.12 | Cytisine | – | 0.43 | 22 | |
(S.D.) | (117) | (0.01) | (73) | (0.05) | (2) |
↵1-a The references are: 1, Anderson and Arneric, 1994; 2,Pabreza et al., 1991; 3, Martino-Barrows and Kellar, 1987; 4, Khan et al., 1994.