Modified Mal II peptides containing d-IIe15inhibited capillary endothelial cell migration
| Peptides | Sequence | ED50 |
|---|---|---|
| nM | ||
| Unmodified Mal II | S P W S S A S V T A G D G V dI T R I R | >200 |
| d-IIe 15-Mal II derivatives | ||
| 19-mer | S P W S S A S V T A G D G V dI T R I R | 8.5 ± 0.7 |
| Nonapeptide | G D G V dI T R I R | 3.0 ± 3.8 |
| Acetylated heptapeptide | ac-G V dI T R I R | 7.1 ± 1.0 |
| Ethylamide-capped heptapeptide | ac-G V dI T R I R-neth | 1.8 ± 0.2 |
| Retro-inverso | cooh-G dV I dT dR dI dR-nh 2 | 7.0 ± 0.6 |
The concentration at which each peptide inhibited 50% of bFGF-stimulated capillary endothelial cell migration was determined from three independent experiments with at least five concentrations of peptide. Each peptide is listed starting from its N terminus, with the exception of the retro-inverso which is listed starting from its C terminus. Ac, acetyl group; Neth, N-ethylamide group.