Abs or mAbs | COL | Retina |
---|---|---|
Anti-α3-COOH | 8 ± 2 | 31 ± 2 |
Anti-α3-CYT | 6 ± 1 | 25 ± 2 |
mAb 313 | 15 ± 3 | 42 ± 1.3 |
Anti-α4-COOH | 86 ± 2 | 45 ± 5 |
Anti-α4-CYT | 45 ± 2 | 25 ± 2 |
mAb 299 | 80 ± 5 | 44 ± 4 |
Anti-α5-COOH | 25 ± 0.9 | 18 ± 3 |
Anti-α5-CYT | 20 ± 0.2 | 14 ± 2 |
mAb35 | 30 ± 1 | 11 ± 3 |
Anti-α6-COOH | 5 ± 1 | 34 ± 3 |
Anti-α6-CYT | 4 ± 0.5 | 35 ± 4 |
Anti-β2-COOH | 93 ± 5 | 45 ± 3 |
Anti-β2-CYT | 89 ± 2 | 30 ± 2 |
mAb 270 | 95 ± 5 | ND |
Anti-β3-COOH | 0.6 ± 0.2 | 28 ± 6 |
Anti-β3-CYT | 5 ± 0.4 | 32 ± 2 |
Anti-β4-COOH | 11 ± 2 | 84.2 ± 1.5 |
Anti-β4-CYT | 13 ± 0.5 | 78 ± 2.5 |
Immunoprecipitation was carried out as described in Materials and Methods using saturating concentrations (20–30 μg) of antisubunit Abs or mAbs. Results are expressed as a percentage of [3H]Epi-labeled receptors, taking the amount of receptor present in the solution before immunoprecipitation as 100%. Percentage of immunoprecipitation was subtracted from value obtained in control samples containing an identical concentration of normal rabbit or rat IgG. Values are mean ± S.E.M. of three determinations.