Table 1

Inhibitory potency of various 15-LOX inhibitors for the pure rabbit 15-LOX

InhibitorInhibition (This Study)IC50Reference
50 nM500 nM
% μM
Ebselen16900.06This study
PD 146 17612590.38This study
Eicosatetraenoic acid7730.12 Schewe et al., 1986
Nordihydroguaiaretic acid9560.5 Schewe et al., 1986
4-Nitrocatechol0544.6 Schewe et al., 1986
tert-Butylhydroxyanisol42160 Schewe et al., 1986
Salicylhydroxymic acid6657 Schewe et al., 1986

The 15-LOX (29 nM) was pre-incubated for 30 s in 1 ml of 0.1 M phosphate buffer (pH 7.4) with different concentrations of the inhibitors. Then linoleic acid (260 μM) was added and the LOX activity was assayed, measuring the increase in absorbance at 235 nm. The numbers represent the means of at least duplicate measurements. It should be stressed that the IC50 values retrieved from the literature cannot be compared directly with the data obtained in this study because different experimental conditions (different LOX preparations, different enzyme concentrations, duration of the preincubation period) were used.