[3H]DAMGO binding in membranes from transfected cells
| Clone | KD | Bmax |
|---|---|---|
| nM | pmol/mg protein | |
| MOR-1 | 1.75 ± 0.44 | 0.16 ± 0.04 |
| MOR-1C | 0.57 ± 0.14 | 0.41 ± 0.07 |
| MOR-1D | 0.35 ± 0.07 | 0.54 ± 0.06 |
| MOR-1E | 0.74 ± 0.11 | 0.25 ± 0.04 |
MOR-1, MOR-1C, MOR-1D, orMOR-1E was transfected into CHO cells, and stable transfectants were isolated. [3H]DAMGO binding was then examined in stable lines expressing either MOR-1 orMOR-1C. Saturation studies were performed and the binding parameters were established by nonlinear regression analysis. Results are the mean ± S.E. of at least three independent determinations.