Table 1

Amino acid sequence motifs in renal organic anion transporter 1 homologs

Gene (species)% Identity with hOAT1Conserved cysteine residuesN-linked glycosylation consensus sitesConsensus sites
PKAPKCCKIITK
hOAT1/ROAT1 49 ,78, 105,128 39, 56 , 92 , 97,113 Ser271 ,Ser278, Ser325, Thr515, Tyr536
 (human) Thr284,Thr334, Ser543
Ser521
rROAT1/OAT188 49 , 78,105, 128 39, 56 , 92 , 97,113 Ser271 ,Ser278, Ser325, Thr515,
 (rat) Thr284,Thr334 Ser544
mNKT (mROAT1)86 49 ,78, 99, 122 39, 56 , 86, 91,107 Ser265 ,Ser272, Ser319, Thr509,
 (mouse) Thr278,Thr328 Ser538
fROAT49 49, 81, 117, 140 54, 95, 124Thr351, Ser409Thr13, Ser168, Ser337, Thr527,
 (winter flounder) Ser283, Ser290, Ser543,
Ser324, Ser536,Thr549
Ser543
  • Potential modification sites for transport proteins with the corresponding amino acid position numbers within their respective polypeptide chains. Boldface denotes positions conserved between homologs, underline denotes positions conserved within mammals, and italics denotes sites unique to hOAT1.