Table 2

Comparison of hOAT1 and rROAT1 substrate affinity

Km μM
  • Each Km was determined from a double reciprocal plot of a 20-min uptake at substrate concentrations ranging from 10 to 400 μM for hOAT1 (PAH was 2 to 20 μM because of its lower Km ) and 50 to 1000 μM for rROAT1. Plotted data were mean ± S.E. values from three to four animals (10 oocytes/concentration/animal), and Km values were rounded to the nearest whole number. Km data of PAH for rROAT1 are reproduced from Sweet et al., 1997.