Receptor | Other Vector | T1/2 of Internalization |
---|---|---|
min | ||
rLHR-wt | pcDNA3.1 | 100 ± 12 |
rLHR-wt | β-Arrestin(319–418) | 161 ± 102-150 |
rLHR-wt | Dynamin-K44A | 215 ± 72-150 |
rLHR-wt | Arrestin-3 | 20 ± 12-150 |
rLHR-L613,614A | pcDNA3.1 | 18 ± 2 |
rLHR-L613,614A | β-Arrestin(319–418) | 118 ± 92-150 |
rLHR-L613,614A | Dynamin-K44A | 231 ± 62-150 |
rLHR-L613,614A | Arrestin-3 | 4 ± 12-150 |
Cells were transiently transfected with the indicated constructs, and the T1/2 values for internalization were measured as described in Materials and Methods.
Each value represents the mean ± S.E.M. of three independent transfections.
↵2-150 p < .05 compared with appropriate vector (i.e., rLHR-wt or rLHR-L613,614A) cotransfected with pcDNA3.1.