Table 2

Effects of arrestin-3 and dominant-negative mutants of nonvisual arrestins and dynamin on the internalization of the125I-hCG-rLHR complex

ReceptorOther VectorT1/2 of Internalization
min
rLHR-wtpcDNA3.1100  ± 12
rLHR-wtβ-Arrestin(319–418)161  ± 102-150
rLHR-wtDynamin-K44A215  ± 72-150
rLHR-wtArrestin-320  ± 12-150
rLHR-L613,614ApcDNA3.118  ± 2
rLHR-L613,614Aβ-Arrestin(319–418)118  ± 92-150
rLHR-L613,614ADynamin-K44A231  ± 62-150
rLHR-L613,614AArrestin-34  ± 12-150

Cells were transiently transfected with the indicated constructs, and the T1/2 values for internalization were measured as described in Materials and Methods.

  • Each value represents the mean ± S.E.M. of three independent transfections.

  • 2-150p < .05 compared with appropriate vector (i.e., rLHR-wt or rLHR-L613,614A) cotransfected with pcDNA3.1.