Effect of leucine mutations on the expression and membrane targeting of the rLHR
| Receptor | 125I-hCG binding | ||
|---|---|---|---|
| Intact Cells | Detergent Extracts | Surface | |
| ng/106cells | % of total | ||
| rLHR-wt | 2.7 ± 0.5 | 4.0 ± 1.0 | 69 ± 12 |
| rLHR-L613,614,615,616A | 1.1 ± 0.4 | 4.2 ± 1.5 | 26 ± 14-150 |
| rLHR-L613,614A | 3.6 ± 1.7 | 5.2 ± 2.0 | 64 ± 6 |
| rLHR-L615,616A | 1.3 ± 0.4 | 3.0 ± 0.4 | 34 ± 34-150 |
| rLHR-L613A | 2.0 ± 0.3 | 4.9 ± 1.7 | 56 ± 4 |
| rLHR-L614A | 3.8 ± 0.5 | 5.2 ± 2.0 | 73 ± 6 |
| rLHR-D611N | 0.6 ± 0.5 | 4.5 ± 2.0 | 10 ± 44-150 |
Cells were transiently transfected with the indicated constructs and used to measure cell surface (i.e., binding to intact cells) and total125I-hCG binding (i.e., binding to detergent extracts) as described in Materials and Methods. The percentage of the binding activity present at the cell surface was calculated by dividing the binding detected in intact cells by that detected in detergent extracts.
Each value represents the mean ± S.E.M. of three or four independent transfections.
↵4-150 p < .05 compared with rLHR-wt.