Effects of steroid treatment on SULT2-40/41 reporter constructs
| SULT2-40/41 Construct | ED50 (95% Confidence Interval) | |
|---|---|---|
| Dexamethasone | Triamcinolone acetonide | |
| nM | ||
| 1938 | 18.1 (8.4–48.0) | 4.95 (1.11–22.1) |
| ΔPstI | 4.57 (0.42–47.4) | 0.121-a |
| 227 | 41.1 (11.6–146) | 3.78 (0.75–19.2) |
| 158 | 2.87 (0.70–11.7) | 1.981-b (0.08–49.9) |
| IR0-Mut | 4.96 (1.50–16.4) | 6.52 (0.58–73.9) |
ED50 estimations for dexamethasone and triamcinolone acetonide-inducible reporter gene expression in primary cultured hepatocytes transiently transfected with SULT2-40/41 5′-luciferase constructs.
Data shown in Figs. 2 and 3 were fit to a sigmoidal function, and ED50 values with 95% confidence intervals were calculated.
↵1-a Because the dose-response relationship for this group was essentially flat, the calculated confidence interval was very wide, 3.68 × 10−29 to 4.17 × 10+26 nM.
↵1-b The response measured at 10−5 M triamcinolone acetonide was excluded from this analysis because the increased response that was seen at this dose was not reproduced in a subsequent experiment.