Cell line | Km | Vmax |
---|---|---|
μM | pmol/cell/h | |
LLC-PK1 | 28.2 ± 4.7 | 16.5 ± 1.7 |
LLCPK1 sis7 | 38.3 ± 5.3 | 11.9 ± 0.1 |
LLCPK MDR1 | 99.4 ± 14.0 | 8.6 ± 1.1 |
Each cell line was incubated with 0.5–200 μM [3H]vinblastine for 4 h. Subsequently, cells were washed and processed for cell-associated radioactivity as described underExperimental Procedures. Data are given as the mean ± S.D. obtained from multiple individual experimental points from two separate experiments. K m is the concentration that gives one-half of the maximal accumulation of vinblastine in the cells;V max is the maximal concentration of vinblastine accumulation in the cells.