Effect of ATP depletion and verapamil on efflux of dolastatin 10 and vinblastine from Burkitt CA46 cells
| Drug | Before Efflux Phase2-a | No Addition2-b | + NaN3/2dG2-c | + Verapamil2-d |
|---|---|---|---|---|
| pmol drug/106 cells ± S.D.2-e | ||||
| Dolastatin 10 | 0.27 ± 0.04 (100) | 0.28 ± 0.01 (104) | 0.28 ± 0.03 (104) | 0.30 ± 0.05 (111) |
| Vinblastine | 0.028 ± 0.006 (100) | 0.0026 ± 0.002 (9.3) | 0.0050 ± 0.003 (18) | 0.0035 ± 0.002 (13) |
The % relative to value before efflux phase is shown in parentheses.
S.D., standard deviation.
↵2-a Cells were incubated for 1 h in medium containing 1.0 nM [3H]dolastatin 10 or [3H]vinblastine. Aliquots were removed, and the cells were centrifuged through silicone oil to obtain the values shown in this column. For the efflux phase of the experiment, the remainder of each culture was divided into three portions, with the cells harvested by centrifugation.
↵2-b Cells were resuspended in medium and incubated for 2 h. Cells were harvested by centrifugation through silicone oil.
↵2-c Cells were resuspended in medium containing NaN3 and 2-deoxy-d-glucose (2dG) and incubated for 2 h. Cells were harvested by centrifugation through silicone oil.
↵2-d Cells were resuspended in medium containing verapamil and incubated for 2 h. Cells were harvested by centrifugation through silicone oil.
↵2-e The experiment was performed twice, and in each experiment data points were based on triplicate samples.