Table 1

Characterization of cell lines stably expressing WT and mutant A3ARs

Cell LineBmaxKd
pmol/mg nM
WT0.90  ± 0.241.90  ± 0.21
Thr307,318,319 → Ala0.57  ± 0.101.81  ± 0.24
Thr307 → Ala0.28  ± 0.071.63  ± 0.17
Thr318,319 → Ala0.82  ± 0.151.69  ± 0.29
Thr318 → Ala1.24  ± 0.201.24  ± 0.14
Thr318 → Glu0.75  ± 0.111.66  ± 0.05
Thr319 → Ala0.39  ± 0.061.73  ± 0.18
Cys302,305 → Ala0.36  ± 0.051.36  ± 0.39

Membranes from CHO cells stably expressing the indicated A3AR cDNA were prepared for use in 125I-ABMECA saturation binding assays as outlined in Experimental Procedures. Data are presented for three to five experiments.