Cell Line | –PABA | +PABA | % Loss of NAT1 Activity |
---|---|---|---|
nmol/min/mg protein | nmol/min/mg protein | ||
THP-1 (confluent)1-a | 22.6 ± 0.6 | 12.1 ± 0.21-150 | 47 ± 7 |
THP-1 (exp. growth) | 26.9 ± 0.2 | 24.3 ± 0.3 | 9 ± 4 |
MCF-71-b | 7.3 ± 0.1 | 3.0 ± 0.11-150 | 59 ± 7 |
Jurkat1-c | 10.1 ± 0.01 | 5.6 ± 0.11-150 | 45 ± 4 |
CEM1-d | 14.8 ± 0.03 | 7.8 ± 0.41-150 | 47 ± 13 |
Cells were grown to confluence (except where indicated) in PABA-free BME medium and then cultured for a further 24 h in the absence or presence of 25 μM PABA. NAT1 activities were measured in cell cytosols and expressed as mean ± S.E. (n = 3).