Binding Parameter | Incubation Temperature | ||
---|---|---|---|
4°C | 22°C | 37°C | |
K d, pM | 9.9 ± 0.8 | 10.6 ± 0.3 | 20 ± 2 |
B max, fmol/mg protein | 162 ± 5 | 167 ± 5 | 166 ± 6 |
Rat brain P2 membrane fractions (5 μg of protein) were incubated in a total volume of 0.3 ml with 1 to 500 pM 5-[125I]iodo-A-85380 for 4 h at 22°C or 37°C, or for 24 h at 4°C. Results were analyzed as described in Experimental Procedures. Data represent mean ± S.E. obtained from three to four experiments per incubation temperature. Experiments were performed in quadruplicate.