Ki values of Y-27632 and Y-30141 for Rho effector kinases and PKCα
| Protein Kinase | Km for ATP | Ki | |
|---|---|---|---|
| Y-27632 | Y-30141 | ||
| μM | μM | ||
| ROCK-I | 0.15 /0.151-a | 0.22 | 0.03 |
| ROCK-II | 0.25 /0.291-a | 0.30 | 0.05 |
| Citron kinase | 0.12 /0.241-a | 5.3 | 1.1 |
| PKN | 0.17 /0.081-a | 3.1 | 1.4 |
| PKCα | 6.1 | 731-b | 6.01-b |
| PKA1-c | 13 | 25 | 0.3 |
| MLCK1-c | 50 | >250 | 14 |
↵1-a Ki values for Y-27632 and Y-30141 were calculated from the Linewaver-Burk analysis based on two series of competition experiments, and the Km values of various kinases for ATP were determined in each experiments, one for competition with Y-27632 and the other for competition with Y-30141, are shown at the left and the right of the slash, respectively.
↵1-b Calculated from IC50 values.
↵1-c Cited from Uehata et al. (1997).