Binding affinity and B max determinations from 125I-MCH saturation binding of stably transfected CHO cell membranes
| MCH Receptor | Kd(nM ± S.E.M.) | Bmax | % WT IP Production |
|---|---|---|---|
| WT | 3.38 ± 1.03 | 75623 ± 13121 | (100) |
| HA-tagged WT | 3.13 ± 0.27 | 91660 ± 13139 | >95@ 1 μM MCH |
| HA-D123A | NO1-a | NO | 0 @10 μM MCH |
↵1-a NO, none observed up to 10 nM125I-MCH.