Table 3

Binding affinity determinations from 125I-MCH/MCH competition binding in stably transfected MCH receptor CHO cell membranes

MCH AnalogIC50 (nM ± S.E.M.)% MCH Ca2+ Response
MCH1.5  ± 0.4(100)
[Ala11]-MCH5630.0  ± 4200NO3-a@ 1μM
[Ala14]-MCH2.7  ± 1.6> 95  @ 1 μM
[Asp11]-MCHNO @ 10 μMNO  @ 10 μM
[Asn11]-MCHNO @ 10 μMNO  @ 1 μM
[d-Arg11]-MCH5100.0  ± 1600NO  @ 10 μM
[Gln11]-MCHNO @ 10 μMNO  @ 10 μM
[Glu11]-MCHNO @ 10 μMNO  @ 1 μM
[Lys11]-MCH197.0  ± 4366.9 ± 12.5 @ 10 μM
  • 3-a NO, none observed at the specified concentrations.