Characteristics of the specific binding of [3H]prazosin to GFP-tagged forms of the hamster α1b-adrenoceptor
| α1b-Adrenoceptor-GFP Construct | [3H]Prazosin | Bmax |
|---|---|---|
| Kd, nM | pmol/mg | |
| Wild type | 0.09 ± 0.02 | 7.0 ± 1.5 |
| 3CAM | 0.12 ± 0.02 | 4.0 ± 0.8 |
| A293E | 0.10 ± 0.02 | 22 ± 4 |
| D142A | 0.10 ± 0.02 | 23 ± 3 |
| M8 | 0.07 ± 0.02 | 11 ± 1 |
The specific binding of various concentrations of [3H]prazosin to membranes of stable clones expressing each of the α1b-adrenoceptor-GFP constructs was measured, and B max and K d values were estimated. Data are presented as mean ± S.E. from three independent experiments.