Table 2

The CB1 cannabinoid receptor might control cell fate through sustained JNK activation

Cell TypeJNK ActivityCell Viability
10-min Incubation72-h Incubation
% %
Primary neurons89  ± 679  ± 1296  ± 7
Primary astrocytes126  ± 112-160 114  ± 10101  ± 8
Glioma C6.9167  ± 182-150 359  ± 1042-150 63  ± 182-150
Glioma C6.4166  ± 202-150 179  ± 102-150 95  ± 6
Astrocytoma U373 MG119  ± 52-160 161  ± 152-150 95  ± 9
CHO-CB1 176  ± 132-150 83  ± 990  ± 4
CHO-wt94  ± 8N.D.104  ± 2

Cells were incubated with or without 1 μM THC for 10 min or 72 h, and JNK activity was measured. Cell viability was also determined in the 72-h incubations. Results are expressed as percentage of the respective incubations with no additions and correspond to three (CHO-CB1, CHO-wt), four (neurons, glioma C6.9, glioma C6.4, astrocytoma U373 MG), or six (astrocytes) experiments.

    • N.D., not determined.

    • Significantly different from incubations with no additions:

    • 2-150P < .01;

    • 2-160P< .05.