Table 1

Photolabeling-induced shifts in affinity for Ro15-1788, zopiclone, and flunitrazepam

LigandControlIrradiatedPhotolabeledPAL/Irradiated Ratio
nM
Ro15-17882.030.97  ± 0.091.48  ± 0.221-150 1.53
Zopiclone29.529.6  ± 2.683.2  ± 6.71-150 2.81
Flunitrazepam6.84.61  ± 1.0235  ± 391-150 51.0

Values are means ± S.E., where n = 6 for Ro15-1788 and n = 3 for zopiclone and diazepam. Values are K d for Ro15-1788 and K i for zopiclone and flunitrazepam.

    • PAL, photolabeled.

    • 1-150P < .01 compared to irradiated samples.